Human iPS to neuron (wt) 1: Difference between revisions
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[12] Okano, Masaki, Daphne W. Bell, Daniel A. Haber, and En Li. "DNA methyltransferases Dnmt3a and Dnmt3b are essential for de novo methylation and mammalian development." Cell 99, no. 3 (1999): 247-257.<br> | [12] Okano, Masaki, Daphne W. Bell, Daniel A. Haber, and En Li. "DNA methyltransferases Dnmt3a and Dnmt3b are essential for de novo methylation and mammalian development." Cell 99, no. 3 (1999): 247-257.<br> | ||
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|germ_layer=ectoderm | |||
|primary_cells=primary cells | |||
|category_treatment=differentiation | |||
|timepoint_design=staged in-vitro diff | |||
|time_span=18 days | |||
|number_time_points=4 | |||
|time_points=day00 | |||
|tissue_cell_type=iPS->neuron | |||
|zenbu_config=http://fantom.gsc.riken.jp/zenbu/gLyphs/#config=6mn9EDwJB9fvyKa00dmd6D | |||
|tet_config=http://fantom.gsc.riken.jp/5/tet/search/?filename=hg19.cage_peak_phase1and2combined_tpm_ann_decoded.osc.txt.gz&file=1&c=1&c=1440&c=1441&c=1442&c=1443&c=1444&c=1445&c=1446&c=1447&c=1448&c=1449&c=1450&c=1451 | |||
}} | }} |
Revision as of 17:27, 3 February 2015
Series: | IN_VITRO DIFFERENTIATION SERIES |
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Species: | Human (Homo sapiens) |
Genomic View: | Zenbu |
Expression table: | FILE |
Link to TET: | [{{{tet_file}}} TET] |
Sample providers : | Christine Wells |
Germ layer: | ectoderm |
Primary cells or cell line: | primary cells |
Time span: | 18 days |
Number of time points: | 4 |
CollapseOverview |
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Human induced pluripotent stem cells can generate every cell type of the human body and under the appropriate conditions recapitulate central aspects of embryonic development in the dish. To interrogate the transcriptome changes associated with the earliest steps of human brain development as recapitulated with human pluripotent stem cells we generated footprint-free induced pluripotent stem cells (iPSC) from control and Down syndrome fibroblasts using episomal reprogramming [1] and were next stepwise differentiated these iPSc into neuro-ectodermal cells (day6), neural stem cells (day12) and early neuronal progenitors (day 18) using an established neuronal differentiation protocol [2]. |
ExpandSample description |
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ExpandQuality control |
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Profiled time course samples
Only samples that passed quality controls (Arner et al. 2015) are shown here. The entire set of samples are downloadable from FANTOM5 human / mouse samples
13421-144D1 | iPS differentiation to neuron, control donor C11-CRL2429 | day00 | rep1 |
13422-144D2 | iPS differentiation to neuron, control donor C11-CRL2429 | day06 | rep1 |
13423-144D3 | iPS differentiation to neuron, control donor C11-CRL2429 | day12 | rep1 |
13424-144D4 | iPS differentiation to neuron, control donor C11-CRL2429 | day18 | rep1 |
13425-144D5 | iPS differentiation to neuron, control donor C11-CRL2429 | day00 | rep2 |
13426-144D6 | iPS differentiation to neuron, control donor C11-CRL2429 | day06 | rep2 |
13427-144D7 | iPS differentiation to neuron, control donor C11-CRL2429 | day12 | rep2 |
13428-144D8 | iPS differentiation to neuron, control donor C11-CRL2429 | day18 | rep2 |
13429-144D9 | iPS differentiation to neuron, control donor C11-CRL2429 | day00 | rep3 |
13430-144E1 | iPS differentiation to neuron, control donor C11-CRL2429 | day06 | rep3 |
13431-144E2 | iPS differentiation to neuron, control donor C11-CRL2429 | day12 | rep3 |
13432-144E3 | iPS differentiation to neuron, control donor C11-CRL2429 | day18 | rep3 |