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Saos calcification

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Series:IN_VITRO DIFFERENTIATION SERIES
Species:Human (Homo sapiens)
Genomic View:Zenbu
Expression table:[{{{tet_config}}} FILE]
Link to TET:[{{{tet_file}}} TET]
Sample providers :Kim Summers
Germ layer:{{{germ_layer}}}
Primary cells or cell line:{{{primary_cells}}}
Time span:{{{time_span}}}
Number of time points:{{{number_time_points}}}


Overview

The formation of bone is a multi-stage process. It begins with mesenchymal cells committed to becoming cartilage. These cells condense into nodules and differentiate into chondrocytes which proliferate rapidly to form the template for the developing bone. They secrete a cartilege-specific extracellular matrix. blood vessels sinvade the cartilage structure. Mesenchymal precursors surrounding the cartilage cells then differentiate into osteoblasts which begin to form an extracelular matrix specific for bone. At the same time the chondrocytes within the osteoblast shell begin to die from apoptosis leaving a space that becomes the bone marrow. osteoblasts themselves become embedded into the bone matrix and differentiate further into osteocytes. Bone is constantly being remodelled by the action of osteoclasts, cells of the monocyte lineage that degrade the bone and osteoblasts on the surface which replace it with new bone. Thus the formation and maintenance of bone is a dynamic process. During the formation of bone, osteoblasts lay down a matrix of hydroxyapatite, a mineral containing calciumand phosphorous which makes up to 50% of the weight of bone. this process of mineralisation can be simulated in vitro in cell cultures treated with chemicals to induce calcification. Many cell lines, primarily derived from osteosarcomas, can be induced to mineralise in this way, as can primary vascular smooth muscle cells. It is important to understand the process of mineralisation because formation of the bony skeleton is critical to the proper functioning of the vertebrate organism and because ectopic mineralisation can occur in genetic and environmental disease. For example, calcification is a key finding in forms of arterial disease including atherosclerosis.

Sample description

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Quality control

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Profiled time course samples

Only samples that passed quality controls (Arner et al. 2015) are shown here. The entire set of samples are downloadable from FANTOM5 human / mouse samples


12662-134I7Saos-2 osteosarcoma treated with ascorbic acid and BGP to induce calcification00hr00minbiol_rep1 (A1 T0)
12663-134I8Saos-2 osteosarcoma treated with ascorbic acid and BGP to induce calcification00hr15minbiol_rep1 (A1 T1)
12664-134I9Saos-2 osteosarcoma treated with ascorbic acid and BGP to induce calcification00hr30minbiol_rep1 (A1 T2)
12665-135A1Saos-2 osteosarcoma treated with ascorbic acid and BGP to induce calcification00hr45minbiol_rep1 (A1 T3)
12666-135A2Saos-2 osteosarcoma treated with ascorbic acid and BGP to induce calcification01hr00minbiol_rep1 (A1 T4)
12667-135A3Saos-2 osteosarcoma treated with ascorbic acid and BGP to induce calcification01hr20minbiol_rep1 (A1 T5)
12668-135A4Saos-2 osteosarcoma treated with ascorbic acid and BGP to induce calcification01hr40minbiol_rep1 (A1 T6)
12669-135A5Saos-2 osteosarcoma treated with ascorbic acid and BGP to induce calcification02hr00minbiol_rep1 (A1 T7)
12670-135A6Saos-2 osteosarcoma treated with ascorbic acid and BGP to induce calcification02hr30minbiol_rep1 (A1 T8)
12671-135A7Saos-2 osteosarcoma treated with ascorbic acid and BGP to induce calcification03hrbiol_rep1 (A1 T9)
12672-135A8Saos-2 osteosarcoma treated with ascorbic acid and BGP to induce calcification04hrbiol_rep1 (A1 T10)
12673-135A9Saos-2 osteosarcoma treated with ascorbic acid and BGP to induce calcification08hrbiol_rep1 (A1 T11)
12674-135B1Saos-2 osteosarcoma treated with ascorbic acid and BGP to induce calcification24hrbiol_rep1 (A1 T12)
12675-135B2Saos-2 osteosarcoma treated with ascorbic acid and BGP to induce calcificationday04biol_rep1 (A1 T13)
12676-135B3Saos-2 osteosarcoma treated with ascorbic acid and BGP to induce calcificationday07biol_rep1 (A1 T14)
12677-135B4Saos-2 osteosarcoma treated with ascorbic acid and BGP to induce calcificationday14biol_rep1 (A1 T15)
12678-135B5Saos-2 osteosarcoma treated with ascorbic acid and BGP to induce calcificationday21biol_rep1 (A1 T16)
12679-135B6Saos-2 osteosarcoma treated with ascorbic acid and BGP to induce calcificationday28biol_rep1 (A1 T17)
12760-136B6Saos-2 osteosarcoma treated with ascorbic acid and BGP to induce calcification00hr00minbiol_rep2 (A2 T0)
12761-136B7Saos-2 osteosarcoma treated with ascorbic acid and BGP to induce calcification00hr15minbiol_rep2 (A2 T1)
12762-136B8Saos-2 osteosarcoma treated with ascorbic acid and BGP to induce calcification00hr30minbiol_rep2 (A2 T2)
12763-136B9Saos-2 osteosarcoma treated with ascorbic acid and BGP to induce calcification00hr45minbiol_rep2 (A2 T3)
12764-136C1Saos-2 osteosarcoma treated with ascorbic acid and BGP to induce calcification01hr00minbiol_rep2 (A2 T4)
12765-136C2Saos-2 osteosarcoma treated with ascorbic acid and BGP to induce calcification01hr20minbiol_rep2 (A2 T5)
12766-136C3Saos-2 osteosarcoma treated with ascorbic acid and BGP to induce calcification01hr40minbiol_rep2 (A2 T6)
12767-136C4Saos-2 osteosarcoma treated with ascorbic acid and BGP to induce calcification02hr00minbiol_rep2 (A2 T7)
12768-136C5Saos-2 osteosarcoma treated with ascorbic acid and BGP to induce calcification02hr30minbiol_rep2 (A2 T8)
12769-136C6Saos-2 osteosarcoma treated with ascorbic acid and BGP to induce calcification03hrbiol_rep2 (A2 T9)
12770-136C7Saos-2 osteosarcoma treated with ascorbic acid and BGP to induce calcification04hrbiol_rep2 (A2 T10)
12771-136C8Saos-2 osteosarcoma treated with ascorbic acid and BGP to induce calcification08hrbiol_rep2 (A2 T11)
12772-136C9Saos-2 osteosarcoma treated with ascorbic acid and BGP to induce calcification24hrbiol_rep2 (A2 T12)
12773-136D1Saos-2 osteosarcoma treated with ascorbic acid and BGP to induce calcificationday04biol_rep2 (A2 T13)
12774-136D2Saos-2 osteosarcoma treated with ascorbic acid and BGP to induce calcificationday07biol_rep2 (A2 T14)
12775-136D3Saos-2 osteosarcoma treated with ascorbic acid and BGP to induce calcificationday14biol_rep2 (A2 T15)
12776-136D4Saos-2 osteosarcoma treated with ascorbic acid and BGP to induce calcificationday21biol_rep2 (A2 T16)
12777-136D5Saos-2 osteosarcoma treated with ascorbic acid and BGP to induce calcificationday28biol_rep2 (A2 T17)
12858-137D5Saos-2 osteosarcoma treated with ascorbic acid and BGP to induce calcification00hr00minbiol_rep3 (A3 T0)
12859-137D6Saos-2 osteosarcoma treated with ascorbic acid and BGP to induce calcification00hr15minbiol_rep3 (A3 T1)
12860-137D7Saos-2 osteosarcoma treated with ascorbic acid and BGP to induce calcification00hr30minbiol_rep3 (A3 T2)
12861-137D8Saos-2 osteosarcoma treated with ascorbic acid and BGP to induce calcification00hr45minbiol_rep3 (A3 T3)
12862-137D9Saos-2 osteosarcoma treated with ascorbic acid and BGP to induce calcification01hr00minbiol_rep3 (A3 T4)
12863-137E1Saos-2 osteosarcoma treated with ascorbic acid and BGP to induce calcification01hr20minbiol_rep3 (A3 T5)
12864-137E2Saos-2 osteosarcoma treated with ascorbic acid and BGP to induce calcification01hr40minbiol_rep3 (A3 T6)
12865-137E3Saos-2 osteosarcoma treated with ascorbic acid and BGP to induce calcification02hr00minbiol_rep3 (A3 T7)
12866-137E4Saos-2 osteosarcoma treated with ascorbic acid and BGP to induce calcification02hr30minbiol_rep3 (A3 T8)
12867-137E5Saos-2 osteosarcoma treated with ascorbic acid and BGP to induce calcification03hrbiol_rep3 (A3 T9)
12868-137E6Saos-2 osteosarcoma treated with ascorbic acid and BGP to induce calcification04hrbiol_rep3 (A3 T10)
12869-137E7Saos-2 osteosarcoma treated with ascorbic acid and BGP to induce calcification08hrbiol_rep3 (A3 T11)
12870-137E8Saos-2 osteosarcoma treated with ascorbic acid and BGP to induce calcification24hrbiol_rep3 (A3 T12)
12871-137E9Saos-2 osteosarcoma treated with ascorbic acid and BGP to induce calcificationday04biol_rep3 (A3 T13)
12872-137F1Saos-2 osteosarcoma treated with ascorbic acid and BGP to induce calcificationday07biol_rep3 (A3 T14)
12873-137F2Saos-2 osteosarcoma treated with ascorbic acid and BGP to induce calcificationday14biol_rep3 (A3 T15)
12874-137F3Saos-2 osteosarcoma treated with ascorbic acid and BGP to induce calcificationday21biol_rep3 (A3 T16)
12875-137F4Saos-2 osteosarcoma treated with ascorbic acid and BGP to induce calcificationday28biol_rep3 (A3 T17)
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